WT M. smegmatis was transfected with either pJAM2 empty vector or pYAB262, a previously published pJAM2-based acetamide-inducible vector to overexpress mptA16 (link). The transformed strains were grown in Middlebrook 7H9 containing 20 µg/mL kanamycin and mptA overexpression was induced with 0.2% acetamide for 8 h.
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