Quantitative Body Composition Analysis

Fat mass and lean mass were measured with an EchoMRI-500 whole body composition analyzer (Echo Medical Systems, LLC, Houston, TX, USA) according to the manufacturer’s instruction 3 days before rats were euthanized. The analyzer measures fat mass, lean mass, and free water in scanned specimens using quantitative magnetic resonance theory, and this method has been validated in rats [13 (link)]. A clear plastic container of canola oil with a similar weight to the experimental rat was placed in the machine and tested as a fat standard before measuring body composition. Individual live rats were placed in a clear holder. The holder was then placed into a tubular space in the EchoMRI system and activated through a software program. Fat is expressed as equivalent weight in canola oil. Lean is the muscle tissue mass equivalent of all body parts containing water, excluding fat, bone minerals, hair, claws, etc. Free water comes mostly from the bladder.

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Cao J.J, & Gregoire B.R. (2024). Calcium Deficiency Decreases Bone Mass without Affecting Adiposity in Ovariectomized Rats Fed a High-Fat Diet. Nutrients, 16(4), 478.

Publication 2024

EchoMRI-500 whole body composition analyzer

Corresponding Organization : Agricultural Research Service

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Fat mass
Lean mass
Free water

control variables

Canola oil with similar weight to the experimental rat was placed in the machine and tested as a fat standard before measuring body composition

controls

Positive control: Canola oil used as a fat standard
Negative control: Not explicitly mentioned

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