Mouse Immune Cell Trafficking Protocol

All experiments were approved by the Institutional Animal Care and Use Committee of Ajou University (IACUC No. 2014-0038). WT C57BL/6 mice were purchased from Charles River Laboratories (Orient Bio Inc., Sungnam, Korea). CX₃CR1^(GFP/+) mice, MyD88 knockout (KO) mice, CD45.1⁺ C57BL/6 mice and CCR7 KO mice (C57BL/6 background) were purchased from Jackson Laboratories (Bar Harbor, ME). CX₃CR1-DTR mice (C57BL/6 background) were kindly provided by Dan R. Littman (New York University Medical Center) and Charles Surh (Pohang University of Science and Technology) (20 (link)). All mice used in the study were at 6 weeks of age. The mice were kept in the Laboratory Animal Research Center of Ajou University Medical Center.