SARS-CoV-2 Infection in K18-hACE2 Mice

SARS-CoV-2 was isolated from
a COVID-19 positive-tested patient.^{64 (link)} We
used K18-hACE2 humanized mice (B6.Cg-Tg(K18-ACE2)2Prlmn/J)^{32 (link),65 (link),66 (link)} from Jackson Laboratory, which
has been used as model for SARS-CoV-2-induced disease.^{65 (link)−71 (link)} Mice were breed in the Centro de Criação de Animais
Especiais (Ribeirão Preto Medical School/University of São
Paulo). Experiments were approved by the University of Sao Paulo ethics
committee (protocol number 105/2021). Mice had access to food and
water ad libitum. Animals were transferred to the BSL3 facility for
the experimental infection.

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Puhl A.C., Gomes G.F., Damasceno S., Fritch E.J., Levi J.A., Johnson N.J., Scholle F., Premkumar L., Hurst B.L., Lee-Montiel F., Veras F.P., Batah S.S., Fabro A.T., Moorman N.J., Yount B.L., Dickmander R.J., Baric R.S., Pearce K.H., Cunha F.Q., Alves-Filho J.C., Cunha T.M, & Ekins S. (2022). Vandetanib Blocks the Cytokine Storm in SARS-CoV-2-Infected Mice. ACS Omega, 7(36), 31935-31944.

Publication 2022

B6.Cg-Tg(K18-ACE2)2Prlmn/J

Ace2 Animals Food Infection Mice Patient Sars cov 2 Sars cov 2 disease

Corresponding Organization : Universidade de São Paulo

Other organizations : University of North Carolina at Chapel Hill, North Carolina State University, Utah State University

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Variable analysis

independent variables

SARS-CoV-2 isolated from a COVID-19 positive-tested patient

dependent variables

SARS-CoV-2-induced disease in K18-hACE2 humanized mice

control variables

K18-hACE2 humanized mice (B6.Cg-Tg(K18-ACE2)2Prlmn/J) from Jackson Laboratory
Mice were bred in the Centro de Criação de Animais Especiais (Ribeirão Preto Medical School/University of São Paulo)
Experiments were approved by the University of Sao Paulo ethics committee (protocol number 105/2021)
Mice had access to food and water ad libitum
Animals were transferred to the BSL3 facility for the experimental infection

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