RANKL and OPG Protein Expression

The primary antibodies used in the present study were against the following antigens: RANKL (rabbit polyclonal antibody, dilution 1 : 1000, Proteintech), OPG (rabbit polyclonal antibody, dilution 1 : 300, Abcam, Cambridge, MA, USA), and β-actin (internal control, rabbit polyclonal antibody, dilution 1 : 5000, Abcam). Proteins from the knee joint were separated using SDS-PAGE then transferred to polyvinylidene difluoride membranes. The intensity of each protein was normalized using β-actin and expressed as a proportion of the control. Analysis of Western blots was performed in accordance with a previous study [21 (link)].

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Zeng L., Li C., Jiang H., Chen Y., Li Z., Xu F, & Liu R. (2021). Total Saponins from Nigella glandulifera Seeds Ameliorate Adjuvant-Induced Rheumatoid Arthritis in Rats by Inhibition of an Inflammatory Response and Bone Erosion. BioMed Research International, 2021, 6613527.

Publication 2021

RANKL β-actin

Actin Antibodies Antibody Antigens Dilution Knee joint Membranes Polyvinylidene difluoride Protein Rabbit Rankl Sds page Western blots analysis

Corresponding Organization : Xinjiang Institute of Materia Medica

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Variable analysis

independent variables

RANKL antibody
OPG antibody

dependent variables

Protein expression levels of RANKL and OPG

control variables

β-actin (internal control)

controls

Positive control: Samples from the knee joint
Negative control: Not specified

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