Zika Virus Surveillance in Miami-Dade Mosquitoes

24,351 Ae. aegypti and Ae. albopictus mosquitoes (sorted into 2,596 pools) were collected throughout Miami-Dade County during June to November, 2016 using BG-Sentinel mosquito traps (Biogents AG). Up to 50 mosquitoes of the same species and sex were pooled per trap. The pooled mosquitoes were stored in RNAlater (Invitrogen), RNA was extracted using either the RNAeasy kit (QIAGEN) or MagMAX for Microarrays Total RNA Isolation Kit (Ambion), and ZIKV RNA was detected by qRT-PCR targeting the envelope protein coding region^{40 (link)} or the Trioplex qRT-PCR kit^{41 (link)}. ZIKV infection rates were calculated per 1,000 female Ae. aegypti mosquitoes using the bias-corrected maximum likelihood estimate (MLE)⁴². Days of insecticide usage by the Miami-Dade Mosquito Control were inferred from the zone-specific ZIKV activities timelines published by the Florida DOH³⁵.

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Grubaugh N.D., Ladner J.T., Kraemer M.U., Dudas G., Tan A.L., Gangavarapu K., Wiley M.R., White S., Thézé J., Magnani D.M., Prieto K., Reyes D., Bingham A., Paul L.M., Robles-Sikisaka R., Oliveira G., Pronty D., Barcellona C.M., Metsky H.C., Baniecki M.L., Barnes K.G., Chak B., Freije C.A., Gladden-Young A., Gnirke A., Luo C., MacInnis B., Matranga C.B., Park D.J., Qu J., Schaffner S.F., Tomkins-Tinch C., West K.L., Winnicki S.M., Wohl S., Yozwiak N.L., Quick J., Fauver J.R., Khan K., Brent S.E., Reiner RC J.r., Lichtenberger P.N., Ricciardi M., Bailey V.K., Watkins D.I., Cone M.R., Kopp EW I.V., Hogan K.N., Cannons A.C., Jean R., Monaghan A.J., Garry R.F., Loman N.J., Faria N.R., Porcelli M.C., Vasquez C., Nagle E.R., Cummings D.A., Stanek D., Rambaut A., Sanchez-Lockhart M., Sabeti P.C., Gillis L.D., Michael S.F., Bedford T., Pybus O.G., Isern S., Palacios G, & Andersen K.G. (2017). Genomic epidemiology reveals multiple introductions of Zika virus into the United States. Nature, 546(7658), 401-405.

Publication 2017

RNAlater RNAeasy kit MagMAX for Microarrays Total RNA Isolation Kit Trioplex qRT-PCR kit

Dade Envelope protein Female Insecticide Isolation Microarrays Mosquitoes Per 1 Timelines Zikv Zikv infection

Corresponding Organization : Scripps Research Institute

Other organizations : United States Army Medical Research Institute of Infectious Diseases, University of Oxford, Fred Hutch Cancer Center, Florida Gulf Coast University, Florida Department of Health, University of Miami, Broad Institute, University of Birmingham, Colorado State University, St. Michael's Hospital, University of Washington, Institute for Health Metrics and Evaluation, Miami Dade College, NSF National Center for Atmospheric Research, Tulane University, Miami-Dade Public Library System, University of Nebraska Medical Center, University of Florida, University of Edinburgh, Harvard University, Center for Systems Biology

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Insecticide usage days by the Miami-Dade Mosquito Control

dependent variables

ZIKV infection rates per 1,000 female Ae. aegypti mosquitoes

control variables

Number of mosquitoes pooled per trap (up to 50 of the same species and sex)
Mosquito species (Ae. aegypti and Ae. albopictus)
Mosquito sex
Mosquito collection location (Miami-Dade County)
Mosquito collection period (June to November, 2016)
Mosquito trapping method (BG-Sentinel mosquito traps)
RNA extraction method (RNAeasy kit or MagMAX for Microarrays Total RNA Isolation Kit)
ZIKV detection method (qRT-PCR targeting envelope protein coding region or Trioplex qRT-PCR kit)

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