Comprehensive Immunofluorescence Staining of Embryos

The protocol used for the immunofluorescence staining of embryos was previously described by our research group (Huang et al., 2019 (link); Li et al., 2019 (link)). The sections were visualized using an LSM 800 laser scanning microscope (ZEISS, Germany). F-actin was labeled with Alexa Fluor™ 488 phalloidin, which was purchased from Invitrogen (Carlsbad, CA, USA). A rabbit anti-gamma H2A.X antibody (phospho-S139), a rabbit anti-Aurora B antibody, DAPI staining solution and a goat anti-rabbit IgG H&L antibody (Alexa Fluor® 488) were obtained from Abcam (Cambridge, UK). An Alexa Fluor® 594-conjugated mouse anti-MAD2 antibody was obtained from Santa Cruz (Dallas, TX, USA). A mouse anti-α-tubulin-FITC monoclonal antibody was procured from Sigma-Aldrich (St. Louis, MO, USA).