In Vivo Spinal Cord Imaging Protocol

The cervical spinal cord was exposed by laminectomy as previously described [38 ]. Briefly, animals were intubated by insertion of a canula in the trachea providing oxygen and isoflurane through a Minivent system (Harvard apparatus). Laminectomy (C2-5) was performed and spinal column fixed at C1 and C6 in a stereotactic frame. Body temperature and heart rate were recorded and stored electronically. Imaging was performed via 2-photon (LaVision TrimScope II microscope, Spectra-Physics laser). Mouse respiration via MiniVent was synchronized to picture acquisition through a triggering device (TrigViFo) reducing imaging artifacts [97 ]. Through an additional toolkit (VivoFollow II) [97 ], this synchronization allowed real-time distortion correction. Tissue displacement was automatically corrected by objective and stage adjustment through a Python script (VivoFollow I) [96 (link)]. Single images, z-stacks and videos were acquired with ImSpector software (LaVision). Raw images were processed with Fiji [82 (link)] and saved as TIF.

Free full text: Click here

Ivan D.C., Berve K.C., Walthert S., Monaco G., Borst K., Bouillet E., Ferreira F., Lee H., Steudler J., Buch T., Prinz M., Engelhardt B, & Locatelli G. (2023). Insulin-like growth factor-1 receptor controls the function of CNS-resident macrophages and their contribution to neuroinflammation. Acta Neuropathologica Communications, 11, 35.

Publication 2023

ImSpector software

Animals Body temperature Canula insertion Cervical spinal cord Device Frame Heart rate Isoflurane Laminectomy Microscope Mouse Oxygen Python Respiration Spinal column Tissue Trachea

Corresponding Organization :

Other organizations : University of Bern, University of Freiburg, University of Zurich

Top 5 similar protocols

Variable analysis

independent variables

Laminectomy (C2-5)

dependent variables

Tissue displacement
Body temperature
Heart rate

control variables

Spinal column fixed at C1 and C6 in a stereotactic frame
Mouse respiration via MiniVent synchronized to picture acquisition through a triggering device (TrigViFo)
Tissue displacement automatically corrected by objective and stage adjustment through a Python script (VivoFollow I)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!