Identification and Characterization of Soybean Hexokinase Genes

HMMER 3.0 software was used to search the soybean genome database for genes containing the structural domain of hexokinase (PF03727 and PF00349) and 17 soybean hexokinase genes were retrieved finally. The HXK gene sequences of G. max was downloaded from Ensembl Plants (http://plants.ensembl.org/index.html). The physical and chemical parameters of the proteins, including molecular weight (MV) and theoretical isoelectric point (PI), were computed by ExPASy (http://web.expasy.org/protparam/). The presence of a chloroplast transit peptide (cTP) in the protein sequence was predicted using the ChloroP 1.1 Server (http://www.cbs.dtu.dk/services/ChloroP/). The existence of transmembrane helices (TMHs) in the protein was predicted using TMHMM Server v. 2.0 (http://www.cbs.dtu.dk/services/TMHMM-2.0/). Chromosomal localizations of GmHXKs were mapped in Map Gene2Chromosome v2 (http://mg2c.iask.in/mg2c_v2.0/).

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Chen S., Tian Z, & Guo Y. (2023). Characterization of hexokinase gene family members in Glycine max and functional analysis of GmHXK2 under salt stress. Frontiers in Genetics, 14, 1135290.

Publication 2023

Chloroplast transit peptide Chromosomal Gene Genome Helices Hexokinase Physical Plants Protein Protein sequence Soybean

Corresponding Organization : Zhengzhou University

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Variable analysis

independent variables

The use of HMMER 3.0 software to search the soybean genome database for genes containing the structural domain of hexokinase (PF03727 and PF00349)

dependent variables

The retrieval of 17 soybean hexokinase genes
The physical and chemical parameters of the proteins, including molecular weight (MV) and theoretical isoelectric point (PI)
The presence of a chloroplast transit peptide (cTP) in the protein sequence
The existence of transmembrane helices (TMHs) in the protein
The chromosomal localizations of GmHXKs

control variables

Control variables not explicitly mentioned.

positive controls

No positive controls specified.

negative controls

No negative controls specified.

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