Macrophages were plated onto 8-chambered coverglass (LabTek, Thermoscientific, Rochester, NY) (13 (link), 22 (link), 23 (link)). Cells were incubated with stimuli at 37°C for specified times. Coverglass were then mounted on Nikon Ti-E inverted microscope equipped with CSU-X1 confocal spinning-disk head (Yokogawa, Sugarland, TX). A coherent, 4 Watt laser (Coherent, Santa Clara, CA) was used as an excitation light source to produce excitation wavelengths 488, 568, and 647 nm using an acoustic optical tunable tuner. To acquire high-quality fluorescence images, a high-magnification, high-numerical aperture objective was used (Nikon, 1003, 1.49 numerical aperture, oil immersion) was used. A polarizer (Nikon, MEN 51941) and Wollaston prisms (Nikon, MBH76190) were used to acquire differential interference contrast (DIC) images. Emission light from the sample was collected after passage through the appropriate emission filters (Semrock, Rochester, NY). Images were acquired using an EM-CCD camera (Hamamatsu, C9100-13, Bridgewater, NJ). Image acquisition was performed using MetaMorph software (Molecular Devices, Dowington, PA). Raw image data files were processed using Adobe Photoshop CS4 and assembled in Adobe Illustrator, version CS4 (Adobe Systems, San Jose, CA).