Influenza Virus Subtypes Propagation

Influenza A Virus (IAV) subtypes, A/England/195/2009 (pH1N1) and the A/HK/1174/99 (H3N2), were obtained as described recently (32 (link), 33 (link)). H1N1 and H3N2 pseudotyped viral particles were produced using the plasmids obtained from Addgene (32 (link), 33 (link)). pcDNA3.1-swineH1-flag (H1 from swine H1N1 A/California/04/09) (Invitrogen), pcDNA3.1-swine N1-flag (N1 from swine H1N1 A/California/04/09), and pcDNA H3 (from A/Denmark/70/03 (H3N2) (Invitrogen) were purchased commercially. Plasmid pI.18-N2 [N2 from A/Texas/50/2012/(H3N2)] was provided by Dr Nigel Temperton (University of Kent). Anti-influenza viral antibodies and recombinant viral proteins were obtained from BEI Resources, National Institutes of Health, USA (32 (link), 33 (link)).

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Varghese P.M., Mukherjee S., Al-Mohanna F.A., Saleh S.M., Almajhdi F.N., Beirag N., Alkahtani S.H., Rajkumari R., Nal Rogier B., Sim R.B., Idicula-Thomas S., Madan T., Murugaiah V, & Kishore U. (2021). Human Properdin Released By Infiltrating Neutrophils Can Modulate Influenza A Virus Infection. Frontiers in Immunology, 12, 747654.

Publication 2021

pcDNA3.1-swineH1-flag pcDNA3.1-swine N1-flag pcDNA H3

Anti antibodies Influenza a virus Influenza viral Plasmid Recombinant proteins Swine Viral particles Viral proteins

Corresponding Organization :

Other organizations : Brunel University of London, Vellore Institute of Technology University, National Institute for Research in Reproductive Health, King Faisal Specialist Hospital & Research Centre, King Saud University, Centre d’Immunologie de Marseille-Luminy, University of Oxford

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Variable analysis

independent variables

Influenza A Virus (IAV) subtypes, A/England/195/2009 (pH1N1) and the A/HK/1174/99 (H3N2)
H1N1 and H3N2 pseudotyped viral particles

dependent variables

Not explicitly mentioned

control variables

Plasmids obtained from Addgene (32, 33)
PcDNA3.1-swineH1-flag (H1 from swine H1N1 A/California/04/09) (Invitrogen)
PcDNA3.1-swine N1-flag (N1 from swine H1N1 A/California/04/09)
PcDNA H3 (from A/Denmark/70/03 (H3N2) (Invitrogen)
Plasmid pI.18-N2 [N2 from A/Texas/50/2012/(H3N2)] provided by Dr Nigel Temperton (University of Kent)
Anti-influenza viral antibodies and recombinant viral proteins obtained from BEI Resources, National Institutes of Health, USA (32, 33)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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