OVCAR8 Xenograft Tumor Model

1–2 × 10⁶ of OVCAR8 cells were counted and prepared as suspensions in 0.5 mL PBS for subcutaneous (flank) injections into 6–8 weeks old athymic nude female mice. Tumors were grown for two weeks before the mice were randomized into treatment groups. Mice then received intraperitoneal (IP) treatment with vehicle control (5% dextrose), Panobinostat 10 mg/kg, per oral (PO) treatment of tolinapant 16 mg/kg, or combination panobinostat plus tolinapant. Body weights and tumor measurements were taken twice weekly for 8–10 weeks or as required by humane endpoints. Subcutaneous tumor volumes were calculated according to the formula V = 1/2(length × width²).