WT or mutated BUB1B-promoter was cloned into the pGL3-Luc reporter vector (Genepharma, Shanghai). LUAD cell lines were plated into 24-well plates and transfected with ZNF143 plasmids using Lipofectamine 2000 (Invitrogen, Waltham, MA, USA) after 24 h. After 48 h, luciferase activity was measured by using the Dual-Luciferase reporter assay system(Genepharma, Shanghai) as the manufacturer’s instructions [20 (link)].
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