Chemical Library Handling and Cell Line Assays

TCDD was obtained from Dr. Steve Safe (Texas A&M University) and was handled and disposed of in accordance with University of California safety policies. The chemical library of 176 compounds obtained from Dr. Bruce Hammock (University of California, Davis) was described previously.^{9 (link)} Fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Lawrenceville, GA), and Geneticin (G418) and Alpha Minimal Essential Medium (α-MEM) were from Invitrogen (San Diego, CA). CALUX cell lines H1L6.1c3 (mouse hepatoma (Hepa1c1c7)), H4L1.1c4 (rat hepatoma (H4IIe)), G16L1.1c8 (guinea pig intestinal adenocarcinoma (GPC-16)), and HG2L6.1c1 (human hepatoma (HepG2)) were previously described.^{22 (link), 23 (link)}

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Brennan J.C., He G., Tsutsumi T., Zhao J., Wirth E., Fulton M.H, & Denison M.S. (2015). Development of Species-Specific Ah Receptor-Responsive Third Generation CALUX Cell Lines with Increased Sensitivity and Responsiveness. Environmental science & technology, 49(19), 11903-11912.

Publication 2015

Fetal bovine serum (FBS) Geneticin (G418) Alpha Minimal Essential Medium (α-MEM)

Adenocarcinoma Alpha minimal essential medium Biologicals Cell lines Fetal bovine serum G418 Geneticin Guinea pig Hepatoma Human Intestinal Mouse Safety Tcdd

Corresponding Organization : University of California, Davis

Other organizations : National Institute of Health Sciences, NOAA National Centers for Coastal Ocean Science

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Variable analysis

independent variables

TCDD (obtained from Dr. Steve Safe, Texas A&M University)
Chemical library of 176 compounds (obtained from Dr. Bruce Hammock, University of California, Davis)

dependent variables

Not explicitly mentioned

control variables

Fetal bovine serum (FBS) from Atlanta Biologicals (Lawrenceville, GA)
Geneticin (G418) and Alpha Minimal Essential Medium (α-MEM) from Invitrogen (San Diego, CA)
CALUX cell lines H1L6.1c3 (mouse hepatoma (Hepa1c1c7)), H4L1.1c4 (rat hepatoma (H4IIe)), G16L1.1c8 (guinea pig intestinal adenocarcinoma (GPC-16)), and HG2L6.1c1 (human hepatoma (HepG2))

controls

No positive or negative controls were explicitly mentioned in the input.

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