GL261 glioma cells (a gift from Geza Safrany, National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary) were cultured in DMEM (Life Technologies) supplemented with 10% FBS (Sigma-Aldrich) at 37°C and 5% CO2/95% air in a humidified chamber.
Human dermal microvascular endothelial cells (HDMECs) or human dermal blood endothelial cells (HDBECs) (PromoCell) were cultured in gelatin-coated culture dishes in Endothelial Cell Basal Medium with full supplements (PromoCell, EBM-MV2) at 37°C and 5% CO2/95% air in a humidified chamber.
Primary mouse brain endothelial cells were isolated from 12-week-old C57BL/6 WT or CD93–/– mice as previously described (48 (link)).
Mycoplasma test was routinely performed in all cell cultures used in this study.
Human dermal microvascular endothelial cells (HDMECs) or human dermal blood endothelial cells (HDBECs) (PromoCell) were cultured in gelatin-coated culture dishes in Endothelial Cell Basal Medium with full supplements (PromoCell, EBM-MV2) at 37°C and 5% CO2/95% air in a humidified chamber.
Primary mouse brain endothelial cells were isolated from 12-week-old C57BL/6 WT or CD93–/– mice as previously described (48 (link)).
Mycoplasma test was routinely performed in all cell cultures used in this study.
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