rFMRP was diluted to 11.7 µm in CK2 kinase buffer (NEBuffer for Protein Kinases, #B6022; New England Biolabs, Ipswich, MA) and incubated with 4 mm ATP with or without active CK2 (New England Biolabs, #P6010). Reactions (25-µl) were performed according to the manufacturer’s protocol (New England Biolabs). Human recombinant FMRP S500 was generated as previously described (Evans and Mihailescu, 2010 (link)).
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