Sera and splenocytes (SC) were collected 2 wks following the last immunization. HER2 IgG was measured by binding to HER2+ SKOV3 cells using flow cytometry and Ab concentrations calculated by regression analysis using mAb TA-1 as the standard(20 (link)). Normal mouse serum or isotype matched mAb was the control. Results were analyzed by student’s t test.
HER2-reactive T cells were enumerated by IFN-γ ELISpot assay (BD Biosciences)(21 (link)). PBL or SC were incubated with recombinant HER2 or Neu protein (ecd-Fc fusion; Sino Biological). Results were expressed as spot forming units per 106 SC and analyzed using student’s t test.
HER2-reactive T cells were enumerated by IFN-γ ELISpot assay (BD Biosciences)(21 (link)). PBL or SC were incubated with recombinant HER2 or Neu protein (ecd-Fc fusion; Sino Biological). Results were expressed as spot forming units per 106 SC and analyzed using student’s t test.
