Cell morphological analysis and cell proliferation assay were performed as described [24] (link). Briefly, cells were seeded in 6-well plates at a density of 1×104 cells/well and cultured in the growth medium overnight at 37°C in a humidified incubator with 5% CO2. The next day, maduramicin (0–1 µg/ml) was added. After incubation for 5 days, images were taken with an Olympus inverted phase-contrast microscope equipped with the Quick Imaging system. The cells were then trypsinized and enumerated using a Z1 Coulter Counter (Beckman Coulter, Fullerton, CA, USA). Cells treated with the vehicle (DMSO) alone served as a control.
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