Plasma Biomarker Analysis in Mice

Blood was collected via the abdominal aorta under isoflurane (Isoflurane Inhalation solution, Mylan Inc) anesthesia without prior fasting. The following parameters were determined in the plasma derived from whole blood collected into lithium heparin anticoagulant, clinical biochemistry analyzer JCA-BM6050 (JEOL, Ltd): total protein, Alb, albumin/globulin ratio, glucose, total cholesterol, phospholipids, triglycerides, total bilirubin, AST, ALT, alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and γ-glutamyl transpeptidase (γ-GTP). Also, a part of the plasma sample was used in an enzyme-linked immunosorbent assay system by PhoenixBio Co, Ltd to measure the human-specific ALT1 (hALT1) concentration as previously reported.^{14 (link)}