Calcium imaging was performed as described elsewhere8 (link),65 (link). A single adult animal that expressed the genetically encoded calcium indicators R-CaMP266 (link) in AFD and GCaMP367 (link) in AIY or an animal that expressed GCaMP3 and tagRFP in AFD was placed on a 10% agar pad on a cover slip with 0.1 µm polystyrene beads (Polysciences, Warrington, PA, USA) and covered with another cover slip for immobilization68 (link). The immobilized animals were placed on a Peltier-based temperature controller (Tokai Hit, Fujinomiya, Japan) on a BX61WI microscope (Olympus, Tokyo, Japan). Red and green fluorescence was separated by the Dual-View optics system (Molecular Devices, Sunnyvale, CA, USA), and images were captured by an ImageEM EM-CCD camera (C9100-13, Hamamatsu Photonics, Japan) at a frame rate of 1 Hz. Excitation pulses were generated by a SPECTRA light engine (Lumencor, Beaverton, OR, USA). Fluorescence intensities were measured using the MetaMorph imaging system (Molecular Devices).
Expression of SLO-2::mCherry was observed with a BX53 upright microscope (Olympus).
Expression of SLO-2::mCherry was observed with a BX53 upright microscope (Olympus).
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