A total of 11 human BTC cell lines were used in this study. SNU-245, SNU-308, SNU-478, SNU-869, SNU-1079, SNU-1196, and SNU-2823 cell lines were obtained from Korean Cell Line Bank, Seoul, Korea. HuCCT1 and TFK-1 cell lines were obtained from RIKEN BioResource Center, Ibaraki, Japan. HER2-amplified human BTC cell lines, SNU-2670 and SNU-2773, were established from tumor tissues from BTC patients using a previously described method [31 (link)].
All the cell lines were maintained in RPMI-1640 culture media supplemented with 10% fetal bovine serum (WELGENE Inc., Gyeongsan, Korea) and 10 μg/mL gentamicin in a humidified atmosphere containing 5% CO2 at 37°C.
All the cell lines were maintained in RPMI-1640 culture media supplemented with 10% fetal bovine serum (WELGENE Inc., Gyeongsan, Korea) and 10 μg/mL gentamicin in a humidified atmosphere containing 5% CO2 at 37°C.
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