HBEC Culturing from Healthy and COPD Donors

Human bronchial epithelial cells (HBECs) from three non-smoking healthy donors and three COPD donors (Lonza, Basel, Switzerland) were cultured in Airway Epithelial Cell Medium (Promocell, Heidelberg, Germany) as previously described.27 (link) Further detail is included in the supplement. Characteristics of HBEC donors are detailed in

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Cane J., Tregidgo L., Thulborn S., Finch D, & Bafadhel M. (2021). Antimicrobial Peptides SLPI and Beta Defensin-1 in Sputum are Negatively Correlated with FEV1. International Journal of Chronic Obstructive Pulmonary Disease, 16, 1437-1447.

Publication 2021

Airway Epithelial Cell Medium

Bronchial Copd Cultured medium Donors Epithelial cell Human Supplement

Corresponding Organization :

Other organizations : University of Oxford, Oxford BioMedica (United Kingdom)

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Variable analysis

independent variables

Smoking status (non-smoking vs. COPD)

dependent variables

Not explicitly mentioned

control variables

Cell type (human bronchial epithelial cells)
Cell culture conditions (Airway Epithelial Cell Medium, Promocell, Heidelberg, Germany)
Cell donors (three non-smoking healthy donors and three COPD donors)

controls

Not specified
Not specified

Annotations

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