The molecular weight of dextran was calculated using a PerkinElmer
Series 200 HPLC system, TSKgel G5000PWxl column (30.0 cm × 7.8
mm) and Series 200 RI detector. Dextran (1 mg/mL) was loaded on the
column and diluted using water with a constant flow rate (0.3 mL/min).
Different standards such as Blue dextran (2 000 000
Da; Sigma, USA) and Industrial dextrans (1 400 000;
788 000; 410 000; 112 000; 365 000 Da;
Sigma, USA) were used for the estimation of the molecular weight of
dextran produced by L. mesenteroides SF3.12 (link)