Antibody Characterization for VAPB and PTPIP51

Rabbit and rat antibodies to VAPB and PTPIP51 were as described [20 (link)]. Mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), mouse anti-hemagglutinin (HA), mouse anti-myc 9B11 and rabbit anti-glycogen synthase kinase-3β (GSK3β) phosphorylated on serine-9 (inactive GSK3β) were from Cell Signalling. Rabbit anti-mitofusin-2, rabbit anti-HA, mouse anti-heat shock protein-60 (HSP60) and mouse anti-neurofilament heavy-chain (NFH) (N52) were from Sigma. Rabbit (sc-7011) and mouse (211) anti-α-synuclein, rabbit anti-translocase of the outer mitochondrial membrane protein-20 (TOM20), rabbit anti-fatty acid coenzyme A ligase long-chain 4 (FACL4) and mouse anti-Sigma-1 receptor were from Santa Cruz Biotechnology. Mouse anti-α-synuclein and mouse anti-calnexin were from BD Biosciences. Rabbit anti-EGFP and mouse anti-β-actin were from Abcam. Rabbit anti-PTPIP51 and chicken anti-MAP2 were from Genetex. Mouse anti-protein disulphide isomerase (PDI) was from Affinity Bioreagents, rabbit anti-myc was from Upstate and rabbit anti-GSK3β was from StressGen.

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Paillusson S., Gomez-Suaga P., Stoica R., Little D., Gissen P., Devine M.J., Noble W., Hanger D.P, & Miller C.C. (2017). α-Synuclein binds to the ER–mitochondria tethering protein VAPB to disrupt Ca2+ homeostasis and mitochondrial ATP production. Acta Neuropathologica, 134(1), 129-149.

Publication 2017

GSK3β) Rabbit anti-mitofusin-2 rabbit anti-HA Mouse anti-α-synuclein mouse anti-calnexin Rabbit anti-EGFP mouse anti-β-actin

Actin Antibodies Calnexin Chicken Fatty acid coenzyme a ligase Glyceraldehyde 3 phosphate dehydrogenase Glycogen synthase kinase 3β Heat shock protein 60 Hemagglutinin Map2 Mitofusin 2 Mouse Neurofilament Protein Protein disulphide isomerase Rabbit Serine Sigma 1 receptor Translocase outer mitochondrial membrane α synuclein

Corresponding Organization :

Other organizations : King's College London, University College London, MRC Laboratory for Molecular Cell Biology

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Variable analysis

independent variables

Rabbit and rat antibodies to VAPB and PTPIP51

dependent variables

Not explicitly mentioned

control variables

Mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH)
Mouse anti-hemagglutinin (HA)
Mouse anti-myc 9B11
Rabbit anti-glycogen synthase kinase-3β (GSK3β) phosphorylated on serine-9 (inactive GSK3β)
Rabbit anti-mitofusin-2
Rabbit anti-HA
Mouse anti-heat shock protein-60 (HSP60)
Mouse anti-neurofilament heavy-chain (NFH) (N52)
Rabbit (sc-7011) and mouse (211) anti-α-synuclein
Rabbit anti-translocase of the outer mitochondrial membrane protein-20 (TOM20)
Rabbit anti-fatty acid coenzyme A ligase long-chain 4 (FACL4)
Mouse anti-Sigma-1 receptor
Mouse anti-α-synuclein
Mouse anti-calnexin
Rabbit anti-EGFP
Mouse anti-β-actin
Rabbit anti-PTPIP51
Chicken anti-MAP2
Mouse anti-protein disulphide isomerase (PDI)
Rabbit anti-myc
Rabbit anti-GSK3β

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