Quantification of GSH and GST Levels

Reported protocols were used to quantify the level of GSH. Previously diluted and homogenized tissue was added with freshly prepared PBS and then with a solution of 5-5ʹ-dithiobis (2-nitrobenzoic acid). The 412 nm wavelength was used to check the absorbance of this solution. Similarly, the levels of GSH S-transferase (GST) were assayed using reported protocol with little modifications. Concisely, the same concentrations of GST and 1-chloro-2,4-dinitrobenzene were mixed and diluted with a 0.1 M solution of PBS (pH 6.5). After serial dilution from tissue homogenate, the absorbance was measured at 340 nm [6 (link)].