Culturing Panc-1 and MiaPaCa-2 Pancreatic Cancer Cells

Human pancreatic cancer cell line Panc-1 and MiaPaCa-2 were kindly provided by Dr. Keping Xie of the University of Texas MD Anderson Cancer Center. All cells were cultured in complete DMEM containing 10% fetal bovine serum (FBS, ThermoFisher, Waltham, MA) with 100 units/mL penicillin and 100 µg/mL streptomycin at 37 °C in 5% CO₂ as previously described^{20 (link)–23 (link)}. Monensin, gemcitabine and erlotinib were obtained from Cayman Chemical (Ann Arbor, MI) and LC Laboratories (Woburn, MA), respectively. Unless indicated, all other reagents were purchased from Sigma-Aldrich (St. Louis, MO) or Thermos Fisher Scientific (Waltham, MA).