FAME standard mixture (C4-C24) and isooctane were purchased from Supelco (UK). Protease (from Bacillus amyloliquefaciens), cellulase (from Trichoderma reesei), Folin-Ciocalteu reagent, 5a-cholestan-3b-ol ($95%), sodium methoxide solution (0.5 M, ACS grade), b-sitosterol ($95%), cholesterol ($99%), squalene ($98%), campesterol (∼65%), stigmasterol (∼95%), a-tocopherol ($96%, HPLC grade), g-tocopherol ($96%, HPLC grade), and d-tocopherol ($90%) standards were purchased from Sigma-Aldrich (UK). The Tri-sil HTP reagent was purchased from Thermo Scientic (UK).
Protocol detail
Lipid Profiling of Natural Standards
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Corresponding Organization : University of Reading
Other organizations : Coventry University, Nanjing University of Finance and Economics
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Variable analysis
independent variables
- FAME standard mixture (C4-C24)
- Isooctane
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: Not mentioned
- Negative control: Not mentioned
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