Analysis of PAF-induced Neutrophil Responses

PAF-induced effects on neutrophils were analyzed in the presence of the subsequently listed pharmacological modulators (their proposed targets are given in brackets): Amiloride [200 μM, 10 min, Na⁺-channels (15 (link))], BIX [5 μM, 30 min, sodium-proton exchanger 1 (NHE1) (38 (link)); Tocris, Wiesbaden, Germany], 5-Nitro-2-(3-phenylpropylamino)benzoic acid [NPPB, 100 μM, 10 min, Cl⁻ channels (39 (link))], and ebselen [10 nM, 30 min, glutathione peroxidase and peroxiredoxin enzyme mimetic and NOX2 inhibitor (40 (link), 41 (link)); Tocris]. Following pre-incubation, samples were divided to obtain paired results and treated as control or stimulated with PAF.

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Hug S., Bernhard S., Stratmann A.E., Erber M., Wohlgemuth L., Knapp C.L., Bauer J.M., Vidoni L., Fauler M., Föhr K.J., Radermacher P., Hoffmann A., Huber-Lang M, & Messerer D.A. (2021). Activation of Neutrophil Granulocytes by Platelet-Activating Factor Is Impaired During Experimental Sepsis. Frontiers in Immunology, 12, 642867.

Publication 2021

Amiloride ebselen

Amiloride Benzoic acid Ebselen Enzyme Glutathione peroxidase Neutrophils Nhe1 Nppb Peroxiredoxin Proton Sodium

Corresponding Organization : University Hospital Ulm

Other organizations : Universität Ulm

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Variable analysis

independent variables

Amiloride, 200 μM, 10 min, Na⁺-channels
BIX, 5 μM, 30 min, sodium-proton exchanger 1 (NHE1)
5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), 100 μM, 10 min, Cl⁻ channels
Ebselen, 10 nM, 30 min, glutathione peroxidase and peroxiredoxin enzyme mimetic and NOX2 inhibitor

dependent variables

not explicitly mentioned

control variables

Control
Stimulated with PAF

Annotations

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