Detailed Reagents and Constructs

PDS was from Cayman Chemical (#18013). 10-NCP (10-(4′-(N-diethylamino)butyl)−2-chlorophenoxazine) was from EMD Millipore ((#925681–41). Hoechst dye was from Santa Cruz Biotechnology (#sc-394039). N-TASQ was synthesized as described (Laguerre et al., 2015 (link); Laguerre et al., 2016 (link); Yang et al., 2017 (link)). Antibodies against LC3 were from MBL (#PD014). Antibodies against ATG7 (D12B11; #8558), β-actin (8H10D10; #3700), and the Anti-FLAG DYKDDDDK M2 tag (D6W5B; #2368) were from Cell Signaling. Mouse antibodies against MAP2c (A-4, #sc-74421) were from Santa Cruz Biotechnology. Antibodies against Htt (mEM48), rabbit IgG(H+L) conjugated with horseradish peroxidase (HRP) (#AP307P), and mouse IgG(H+L) conjugated with HRP (#AP308P) were from EMD Millipore. Anti-mouse Alexa Fluor 488-labeled (#A11001) and anti-rabbit Alexa Fluor 546-labeled (#A11010) secondary antibodies were from Life Technologies. A single-chain BG4 antibody that recognizes G4 structures (Biffi et al., 2013 (link)) was purified in the lab of Dr. Nayun Kim. pGW1-Dendra2-LC3 was described (Tsvetkov et al., 2013b (link)). pGW1-mito-Keima was cloned from the mt-mKeima/pIND(SP1) construct that was received from Dr. Atsushi Miyawaki (RIKEN Brain Science Institute, Japan). pCAG-TagBFP, pCAG-EGFP-PIF1, pCAG-EGFP-mPIF1 (E307Q George et al., 2009 (link)), and pEF1A-mApple-ATG7 were cloned by VectorBuilder.

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Moruno-Manchon J.F., Lejault P., Wang Y., McCauley B., Honarpisheh P., Morales Scheihing D.A., Singh S., Dang W., Kim N., Urayama A., Zhu L., Monchaud D., McCullough L.D, & Tsvetkov A.S. (2020). Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons. eLife, 9, e52283.

Publication 2020

10-NCP Hoechst dye AP307P AP308P Anti-mouse Alexa Fluor 488-labeled

Actin Alexa fluor 488 Alexa fluor 546 Antibodies Antibody Brain Cayman Horseradish peroxidase Mito Mouse Pif1 Rabbit Tasq

Corresponding Organization :

Other organizations : Centre National de la Recherche Scientifique, Baylor College of Medicine, The University of Texas Health Science Center at Houston

Top 5 similar protocols

Variable analysis

independent variables

PDS from Cayman Chemical (#18013)
10-NCP (10-(4′-(N-diethylamino)butyl)−2-chlorophenoxazine) from EMD Millipore (#925681–41)
Hoechst dye from Santa Cruz Biotechnology (#sc-394039)
N-TASQ synthesized as described (Laguerre et al., 2015; Laguerre et al., 2016; Yang et al., 2017)

dependent variables

Outcomes related to the use of the specified chemicals and antibodies, but not explicitly mentioned

control variables

Antibodies against LC3 from MBL (#PD014)
Antibodies against ATG7 (D12B11; #8558), β-actin (8H10D10; #3700), and the Anti-FLAG DYKDDDDK M2 tag (D6W5B; #2368) from Cell Signaling
Mouse antibodies against MAP2c (A-4, #sc-74421) from Santa Cruz Biotechnology
Antibodies against Htt (mEM48), rabbit IgG(H+L) conjugated with horseradish peroxidase (HRP) (#AP307P), and mouse IgG(H+L) conjugated with HRP (#AP308P) from EMD Millipore
Anti-mouse Alexa Fluor 488-labeled (#A11001) and anti-rabbit Alexa Fluor 546-labeled (#A11010) secondary antibodies from Life Technologies
A single-chain BG4 antibody that recognizes G4 structures (Biffi et al., 2013) purified in the lab of Dr. Nayun Kim
PGW1-Dendra2-LC3 described (Tsvetkov et al., 2013b)
PGW1-mito-Keima cloned from the mt-mKeima/pIND(SP1) construct received from Dr. Atsushi Miyawaki (RIKEN Brain Science Institute, Japan)
PCAG-TagBFP, pCAG-EGFP-PIF1, pCAG-EGFP-mPIF1 (E307Q George et al., 2009), and pEF1A-mApple-ATG7 cloned by VectorBuilder

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