Thirty SAMP8 mice were divided into three groups (n = 10 per group): the AD group, the MA group, and the medicine (M) group. Ten SAMR1 mice were used as the normal control (N) group.
In the MA group, the mice were immobilized in mouse bags. MA on Baihui (GV20) and Yintang (GV29) was applied for 20 min, with transverse puncturing at a depth of 2–3 mm. The disposable sterile acupuncture needles (0.25 mm × 13 mm) (Beijing Zhongyan Taihe Medicine Company, Ltd.) were used. During the MA on Baihui (GV20) and Yintang (GV29), twirling manipulation was applied every 5 min and lasted 15 s each time. Each needle was rotated bidirectionally within 90° at a speed of 180°/s. For the M group, donepezil hydrochloride tablets (Eisai China, Inc., H20050978) were crushed and dissolved in distilled water and were delivered to mice by oral gavage at a dose of 0.65 μg/g (Geerts et al., 2005 (link)). The above treatments were administered once a day for 15 consecutive days, with no treatment of the N or AD groups. The mice in the N, AD, and M groups received the same 20 min restriction as the MA group. The duration of MA and selection of the acupoints was based on findings from our previous studies (Jiang et al., 2015 (link), 2016 (link), 2018 (link); Cao et al., 2017 (link); Ding et al., 2017 (link)). The above intervention lasted throughout the Morris water maze test period.
In the MA group, the mice were immobilized in mouse bags. MA on Baihui (GV20) and Yintang (GV29) was applied for 20 min, with transverse puncturing at a depth of 2–3 mm. The disposable sterile acupuncture needles (0.25 mm × 13 mm) (Beijing Zhongyan Taihe Medicine Company, Ltd.) were used. During the MA on Baihui (GV20) and Yintang (GV29), twirling manipulation was applied every 5 min and lasted 15 s each time. Each needle was rotated bidirectionally within 90° at a speed of 180°/s. For the M group, donepezil hydrochloride tablets (Eisai China, Inc., H20050978) were crushed and dissolved in distilled water and were delivered to mice by oral gavage at a dose of 0.65 μg/g (Geerts et al., 2005 (link)). The above treatments were administered once a day for 15 consecutive days, with no treatment of the N or AD groups. The mice in the N, AD, and M groups received the same 20 min restriction as the MA group. The duration of MA and selection of the acupoints was based on findings from our previous studies (Jiang et al., 2015 (link), 2016 (link), 2018 (link); Cao et al., 2017 (link); Ding et al., 2017 (link)). The above intervention lasted throughout the Morris water maze test period.
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