Arsenite-Induced Stress Granule Formation

To induce SG formation in cell cultures, sodium arsenite (NaAsO 2 , Sigma-Aldrich S7400) was added to the media at final concentrations of 250 or 500 μM and incubated for 30 minutes. For ex vivo retinal SG induction, eyeballs were dissected and subjected to intravitreal injection with 2 μl of 50 mM arsenite or vehicle (sterile MQ H 2 O), followed by a 30-minute incubation at 37°C in 10% FBS-DMEM.
Subsequently, the eyes were fixed and processed for IHC.

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Benedetto M.M., Malcolm M., Bruera M.G., Penazzi L.G., Guido M.E., Contín M.A., & Garbarino‐Pico E. (2024). Stress granule induction in rat retinas damaged by constant LED light.

Publication 2024

Corresponding Organization : Universidad Nacional de Córdoba

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Variable analysis

independent variables

Sodium arsenite (NaAsO2) concentration (250 μM or 500 μM)
Intravitreal injection of 50 mM arsenite or vehicle (sterile MQ H2O)

dependent variables

SG formation in cell cultures
SG induction in ex vivo retinal tissue

control variables

Incubation time (30 minutes)
Cell culture media composition (10% FBS-DMEM)
Incubation temperature (37°C)

controls

Negative control: Sterile MQ H2O intravitreal injection

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