Electron Microscopy of Plant Stomata

After 4 h under experimental conditions, mature leaves at the same position from each selected plant were excised and immediately cut into small pieces. Then, they were fixed with a 4% glutaraldehyde solution and maintained at 4 °C for 12 h. Stomata on the leaves were observed with a JSM-6360LV scanning electron microscope (SEM; JEOL Ltd., Tokyo, Japan) as previously described^{61 (link)}. Chloroplasts and autophagosomes were observed on a JEOL-1230 transmission electron microscope (TEM, Hitachi, Japan) as described before^{23 (link),49 (link)}.

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Huo L., Sun X., Guo Z., Jia X., Che R., Sun Y., Zhu Y., Wang P., Gong X, & Ma F. (2020). MdATG18a overexpression improves basal thermotolerance in transgenic apple by decreasing damage to chloroplasts. Horticulture Research, 7, 21.

Publication 2020

JSM-6360LV scanning

Autophagosomes Chloroplasts Glutaraldehyde H conditions Plant Scanning electron microscope Stomata Transmission electron microscope

Corresponding Organization :

Other organizations : Northwest A&F University, Nanjing Agricultural University

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Variable analysis

independent variables

Experimental conditions (not explicitly mentioned)

dependent variables

Stomata on the leaves observed with a scanning electron microscope (SEM)
Chloroplasts and autophagosomes observed on a transmission electron microscope (TEM)

control variables

Leaf position on the selected plants
Fixation with 4% glutaraldehyde solution
Maintenance at 4 °C for 12 h

controls

Positive control: Not mentioned
Negative control: Not mentioned

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