T80 cells were grown in RPMI Medium supplemented with 10% FBS. HeLa, 293T, U2OS cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS. RPE1 cells were grown in DMEM F12 media supplemented with 10% FBS. RNF2 knockout T80 clones were generated using the CRISPR-Cas9 Double Nickase plasmid synthesized by Santa Cruz Biotechnology. U2OS cells stably expressing mCherry-LacI-Fok1 fusion protein that induces a DSB at a single genomic locus is previously described [58 (link)]. pyCAG_RNaseH1_WT and D210N plasmids were a gift from Dr. Xiang-Dong Fu (Addgene plasmids #111906, # 111904). Hydroxyurea (AC151680050), Aphidicolin (61197–0010) and DRB (NC9855607) were purchased from Fisher Scientific. alpha-amanitin was purchased from Santa Cruz Biotechnology (sc-202440). IdU (I7125) and CldU (C6891) were purchased from Sigma Aldrich. ATR inhibitor (AZ20) was purchased from SelleckChem.
Free full text: Click here
