LV sections were fixed in a 10% formalin solution, dehydrated in ethanol, and then embedded in paraffin as previously described36 (link). Tissue sections (5 μm) were obtained in a microtome, were deparaffinized, rehydrated, and stained with Masson’s trichrome staining kit (EMD Millipore Corporation, Billerica, MA, USA) and Sirius red staining (Sigma- Aldrich, San Luis, MI, USA) for fibrosis quantification. Hematoxylin/Eosin staining was performed to explore heart morphology (Sigma-Aldrich, San Luis, MI, USA).
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