Vaginal Bacterial DNA Extraction

Gynecologists used two sterile swabs and a speculum to collect vaginal secretions. The swabs were transferred to a − 80 °C refrigerator, and saved for bacterial nucleic acid extraction. Following the standard procedures in our laboratory, [14 (link)] swabs were eluted in 2 ml PBS, and TIANamp Bacteria DNA Kits (Tiangen Biochemical Technology, Beijing, China) were used to extract and purify DNA. The concentrations of DNA were measured through Nanodrop one (Thermo Scientific Co.,Ltd., MA, USA). All the nucleic acid samples were taken successfully, the average concentration is 63.46 ± 10.26 ng/ul.

Free full text: Click here

Hong X., Yin J., Wang W., Zhao F., Ding X., Yu H., Zhang X, & Wang B. (2022). The associations between low abundance of Mycoplasma hominis and female fecundability: a pregnancy-planning cohort study. BMC Microbiology, 22, 121.

Publication 2022

TIANamp Bacteria DNA Kits Nanodrop one

Bacteria dna Bacterial Gynecologists Nucleic acid Secretions Speculum Sterile Vaginal

Corresponding Organization : Development Research Center

Other organizations : Zhongda Hospital Southeast University

Top 5 similar protocols

Variable analysis

independent variables

Sterile swabs used to collect vaginal secretions

dependent variables

Bacterial nucleic acid extraction
DNA concentration

control variables

Maintaining samples at -80°C
Following standard laboratory procedures

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!