Cloning and Sequencing of CYP51 Genes

The amplified CtCYP51 full-length fragments (primers are listed in Supplementary Table S3) were purified using the EasyPure Quick Gel Extraction Kit (TransGen, Beijing, China) and were cloned into the pEASY-T1 simple plasmid (TransGen, Beijing, China) according to the manufacturer’s recommendations. The vector that inserted into Escherichia coli strain was sequenced (Sunbiotech Co., Beijing, China) using vector primers M13F (5′-ACTGGCCGTCGTTTTAC-3′) and M13R (5′-GTCCTTTGT CGATACTG-3′) (Pang et al., 2013 (link); Fan et al., 2014 (link)). DNA sequences were analyzed with DNAMAN5.2.2.0 (Lynnon Biosoft, Quebec, QC, Canada).

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Zhang C., Diao Y., Wang W., Hao J., Imran M., Duan H, & Liu X. (2017). Assessing the Risk for Resistance and Elucidating the Genetics of Colletotrichum truncatum That Is Only Sensitive to Some DMI Fungicides. Frontiers in Microbiology, 8, 1779.

Publication 2017

EasyPure Quick Gel Extraction Kit pEASY-T1 simple plasmid

Dna sequences Escherichia coli Plasmid Primers Strain Vector

Corresponding Organization : China Agricultural University

Other organizations : Chinese Academy of Sciences, University of Maine

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Variable analysis

independent variables

Amplified CtCYP51 full-length fragments

dependent variables

DNA sequences

control variables

PEASY-T1 simple plasmid
Escherichia coli strain
M13F and M13R vector primers

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