Isolation and Characterization of Pancreatic CSCs

NVP-LDE225 and NVP-BEZ-235 were obtained from Chemie Tek (Indianapolis, IN). Antibodies used against Gli1, Gli2, phospho-Akt, Akt, caspase-3, PARP, E-Cadherin, N-Cadherin, Vimentin, Sox-2, Oct-4, Nanog, c-Myc, phospho-Akt, phospho-PI3K, phospho-p70S6K, phospho-GSK3Kβ, phospho-4EBP1, Akt, p70S6K, GSK3Kβ, 4EBP1, Slug, snail, snail, patched 1, patched2, Smo, Sufu Bcl-XL, Bax, Bak, Bcl2, and β-actin were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Enhanced chemiluminescence (ECL) Western blot detection reagents were purchased from Thermo Fisher Scientific Corporation (Waltham, MA).
Isolation and characterization of CD44⁺CD24⁺ESA⁺ pancreatic CSCs have been described elsewhere [54 (link)]. Pancreatic CSCs were grown in well-defined stem cell culture medium [54 (link)–56 (link)].