Mitochondrial and ROS Profiling in Macrophages

Through immunofluorescence staining, we evaluated mitochondrial membrane potential (MMP) and ROS levels in the macrophages isolated from the peritoneal cavity of the experimental and WT mice. As in our previous study [18 (link)], the ROS level was estimated using dichlorofluorescein diacetate (Molecular Probes, Eugene, OR, USA) as an oxidative fluorescent probe, whereas MMP was estimated using the lipophilic cationic fluorescent dye 3,3′-dihexyloxacarbocyanine iodide (Sigma-Aldrich). The mean fluorescent intensity was analyzed using a flow cytometer (FACS Calibur; BD Biosciences PharMingen, San Diego, CA, USA).

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Lu C.W., Wu W.J., Nguyen T.K., Shen S.C., Wu Y.B., Liang H.J, & Wu C.H. (2023). Alleviating Effects of Ovatodiolide and Antcin K Supplements on High-Fat Diet-Induced Cardiovascular Dysfunction in ApoE-Knockout Mice by Attenuating Oxidative Stress. Nutrients, 15(18), 4074.

Publication 2023

dichlorofluorescein diacetate 3,3′-dihexyloxacarbocyanine iodide FACS Calibur

Cationic Fluorescent dye Fluorescent probe Immunofluorescence Iodide Macrophages Mice Mitochondrial membrane potential Molecular probes Peritoneal cavity

Corresponding Organization : National Taiwan Normal University

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Variable analysis

independent variables

Experimental mice
WT (wild-type) mice

dependent variables

Mitochondrial membrane potential (MMP)
Reactive oxygen species (ROS) levels

control variables

Peritoneal macrophages isolated from mice

controls

Positive control: Not specified
Negative control: Not specified

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