Induction of Experimental Autoimmune Encephalomyelitis

Desiccated, killed mycobacterium tuberculosis H37Ra was obtained from Bioscientific Pty Ltd. Complete Freund’s adjuvant (CFA) was purchased from Sigma-Aldrich (MO, USA). Myelin oligodendrocyte glycoprotein (MOG_35-55; amino acid [10 ] sequence: H-Met-Glu-Val-Gly-Trp-Tyr-Arg-Ser-Pro-Phe-Ser-Arg-Val-Val-His-Leu-Tyr-Arg-Asn-Gly-Lys-OH) and pertussis toxin were obtained from Sapphire Biosciences. Interchangeable syringes were obtained from Cadence Inc (VA, USA). 1-Methyl-DL-tryptophan (Sigma-Aldrich, MO, USA) was diluted in 5N HCl and adjusted to pH 7 before injection [11 (link)]. Ro 61-8048 (Tocris, Bristol, UK) was dissolved in DMSO and diluted in sterile 0.9% saline to a concentration of 10 mg/ml, and the pH was adjusted to 7.5 [12 (link)].