Immunohistochemical Detection of VEGF and Amyloid-β

This procedure was performed by a standard indirect method as described previously [8 (link)]. Primary antibodies and dilutions were as follows: anti-VEGF (1:250; # ab14078, Abcam) and anti-amyloid-β_1–42 (1:250; # ab10148, Abcam). After incubation with the respective secondary antibodies conjugated with Alexa Fluor 555 or Alexa Fluor 488 (## ab150170, ab150073, Abcam) diluted 1:250, the tissue slices were coverslipped with the Fluoro-shield mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI; # ab104139, Abcam) and examined under an Axioplan 2 microscope (Zeiss).

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Stefanova N.A., Maksimova K.Y., Rudnitskaya E.A., Muraleva N.A, & Kolosova N.G. (2018). Association of cerebrovascular dysfunction with the development of Alzheimer’s disease-like pathology in OXYS rats. BMC Genomics, 19(Suppl 3), 75.

Publication 2018

ab14078 ab10148 ab150170 ab150073 ab104139 Axioplan 2 microscope

Alexa fluor 488 Alexa fluor 555 Amyloid Antibodies Dapi Dilutions Microscope Tissue Vegf

Corresponding Organization : Institute of Cytology and Genetics

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Variable analysis

independent variables

Primary antibodies: anti-VEGF (1:250) and anti-amyloid-β1–42 (1:250)

dependent variables

Expression levels of VEGF and amyloid-β1–42

control variables

Tissue slices
Secondary antibodies conjugated with Alexa Fluor 555 or Alexa Fluor 488 (diluted 1:250)
Fluoro-shield mounting medium containing DAPI

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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