Protocol detail

Protein Expression Analysis of HK-2 Cells

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HK-2 cells were cultured in cellular six-well plates. Cellular proteins from different subgroups were extracted for WB electrophoresis as described in the previous research (Liu et al., 2022 (link)). The antibodies applied in this research are listed as follows: APOC1(Abcam, United States, 1:1,000 dilution), GAPDH (Proteintech Group, China, 1:4,000 dilution), a-SMA, vimentin, N-cadherin and E-cadherin (Proteintech Group, China, 1:1,000 dilution), NF-κB, and p-NF-κB (CST, USA, 1:1,000 dilution).

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Yu K., Ding L., An X., Yang Y., Zhang X., Li L., Wang C., Bai F, & Yang X. (2023). APOC1 exacerbates renal fibrosis through the activation of the NF-κB signaling pathway in IgAN. Frontiers in Pharmacology, 14, 1181435.

Publication 2023

APOC1 GAPDH a-SMA vimentin N-cadherin E-cadherin

Antibodies Apoc1 Cells Cultured cellular Different cellular Dilution E cadherin Electrophoresis Gapdh N cadherin Nf κb Proteins Vimentin

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Variable analysis

independent variables

Different subgroups of cellular proteins

dependent variables

Expression levels of APOC1, GAPDH, α-SMA, vimentin, N-cadherin, E-cadherin, NF-κB, and p-NF-κB

control variables

HK-2 cells were cultured in cellular six-well plates

controls

Positive control: None specified
Negative control: None specified

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