Cardiometabolic Biomarker Assessment

Serum fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), high-density lipoprotein cholesterol (HDL-c), free fatty acids (FFAs), lipoprotein(a) [Lp(a)], uric acid (UA), and high-sensitivity C reactive protein (hsCRP) were measured by routine automated laboratory methods. Serum insulin, fibroblast growth factor 21 (FGF21), glucagon-like peptide 1 (GLP-1), peptide YY (PYY), and leptin levels were measured by ELISA kits (CEA448Ra, CEC918Ra, CEA804Mi, CEB067Ra, and SEA084Ra, Wuhan USCN Business Co., Ltd., Wuhan, China) following the instructions of the manufacturers. The coefficients of variation for intraassay of insulin, FGF21, GLP1, PYY, and leptin were 2.9, 3.1, 2.0, 1.9, and 3.0%, respectively. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated according to the following formula: fasting serum insulin (pmol/L) × FBG (mmol/L)/135 (Yan et al., 2017 (link)).

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Yan K., Chen W., Zhu H., Lin G., Pan H., Li N., Wang L., Yang H., Liu M, & Gong F. (2018). Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21. Frontiers in Physiology, 9, 191.

Publication 2018

CEA448Ra

Blood glucose Cholesterol Elisa Fgf21 Free fatty acids Glp1 High density lipoprotein cholesterol High sensitivity c reactive protein Homeostasis Insulin Insulin resistance Leptin Low density lipoprotein cholesterol Peptide yy Serum Triglycerides Uric acid

Corresponding Organization :

Other organizations : National Health and Family Planning Commission, Peking Union Medical College Hospital

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Variable analysis

independent variables

Serum fasting blood glucose (FBG)
Total cholesterol (TC)
Triglycerides (TG)
Low-density lipoprotein cholesterol (LDL-c)
High-density lipoprotein cholesterol (HDL-c)
Free fatty acids (FFAs)
Lipoprotein(a) [Lp(a)]
Uric acid (UA)
High-sensitivity C reactive protein (hsCRP)
Serum insulin
Fibroblast growth factor 21 (FGF21)
Glucagon-like peptide 1 (GLP-1)
Peptide YY (PYY)
Leptin levels

dependent variables

Homeostasis model assessment of insulin resistance (HOMA-IR)

control variables

Coefficients of variation for intraassay of insulin, FGF21, GLP1, PYY, and leptin

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