Cloning and injections were performed using established protocols [14 (link)]. A pair of gRNAs designed to delete the region 2R:18,380,931 to 18,391,053 were cloned into pU6-BbsI-chiRNA (Addgene plasmid # 45946). Homology arms (766bp and 840bp left and right, respectively) were cloned into pDsRed-attP (Addgene plasmid # 51019). pBS-Hsp70-Cas9 (Addgene plasmid # 46294) was used as the Cas9 source. Constructs were injected into w1118 flies.
Protocol detail
Genome Editing in Drosophila Using CRISPR
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Arms
Flies
Hsp70
Plasmid
Corresponding Organization :
Other organizations : University of California, San Diego
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Variable analysis
independent variables
- Cloning and injecting gRNAs designed to delete the region 2R:18,380,931 to 18,391,053
- Cloning homology arms (766bp and 840bp left and right, respectively) into pDsRed-attP
- Using pBS-Hsp70-Cas9 as the Cas9 source
- Not explicitly mentioned
- Using w^1118 flies
- No positive or negative controls were explicitly mentioned.
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