Quantifying Epithelial Cell Proliferation

Cell proliferation was assessed using antigen-retrieved paraffin sections co-stained with an antibody against the proliferation marker Ki-67 and DAPI. Images were captured using Abberior Facility Line (Abberior Instruments GmbH, Göttingen, Germany). Channels were merged, and signal was evaluated using Fiji/ImageJ software. The number of Ki-67–positive epithelial nuclei per visual field (40× magnification) [49 (link),50 (link)] were counted. About ten randomly chosen areas in renal cortex were analyzed.

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Dahlke E., Engmann T., Anan Y., Häsler R., Solinas G, & Theilig F. (2022). Tubular IKKβ Deletion Alleviates Acute Ischemic Kidney Injury and Facilitates Tissue Regeneration. International Journal of Molecular Sciences, 23(17), 10199.

Publication 2022

Abberior Facility Line

Antibody Antigen Cell proliferation Dapi Nuclei Paraffin Renal cortex

Corresponding Organization : University of Fribourg

Other organizations : University Hospital Schleswig-Holstein, University of Lübeck, University of Gothenburg

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Number of Ki-67–positive epithelial nuclei per visual field (40× magnification)

control variables

Paraffin sections
Co-staining with an antibody against the proliferation marker Ki-67 and DAPI
Imaging using Abberior Facility Line (Abberior Instruments GmbH, Göttingen, Germany)
Signal evaluation using Fiji/ImageJ software
Randomly chosen areas in renal cortex

controls

None explicitly mentioned

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