Lung Cancer Cell Line Characterization

Lung cancer cell lines were obtained from ATCC or originators and were provided by the Moffitt SPORE in Lung Cancer Cell Core facility. All lines are authenticated by genotyping and maintained free of Mycoplasma. SCLC cell lines were grown in RPMI 1640 with 10% FBS and 1% pen/strep. NSCLC cell lines were cultured in RPMI 1640 with 10% FBS without antibiotics. HLM006474 was synthesized and validated by the Moffitt Chemistry Core as previously described [36] (link). Gemcitabine (Gemzar, Eli Lilly) was purchased through the Moffitt Pharmacy and dissolved in water. Cisplatin and paclitaxel were purchased from Sigma and concentrated stock solutions were prepared in dimethyl sulfoxide.