To isolate cells from colon tissue, colons were isolated, cleared of feces, and prepared according to manufacturer’s instructions with the lamina propria dissociation kit (Miltenyi Biotec) and gentleMACS device (Miltenyi Biotec, program 37C_mLPDK_1). More-detailed protocols about T cell isolation from murine tissues are published (50 (link)) and described in SI Appendix.