Isolation of Colon Cells for Analysis

To isolate cells from colon tissue, colons were isolated, cleared of feces, and prepared according to manufacturer’s instructions with the lamina propria dissociation kit (Miltenyi Biotec) and gentleMACS device (Miltenyi Biotec, program 37C_mLPDK_1). More-detailed protocols about T cell isolation from murine tissues are published (50 (link)) and described in SI Appendix.

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Bittner S., Ruhland B., Hofmann V., Schmidleithner L., Schambeck K., Pant A., Stüve P., Delacher M., Echtenacher B., Edinger M., Hoffmann P., Rehli M., Gebhard C., Strieder N., Hehlgans T, & Feuerer M. (2022). Biosensors for inflammation as a strategy to engineer regulatory T cells for cell therapy. Proceedings of the National Academy of Sciences of the United States of America, 119(40), e2208436119.

Publication 2022

lamina propria dissociation kit gentleMACS device

Cell isolation Cells Colons Device Feces Lamina propria Murine Tissues

Corresponding Organization :

Other organizations : Leibniz Association, University of Regensburg, Johannes Gutenberg University Mainz, University Hospital Regensburg

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Variable analysis

independent variables

Isolation of cells from colon tissue

dependent variables

Isolation of T cells from murine tissues

control variables

Clearing of feces from colon
Preparation of cells according to manufacturer's instructions with the lamina propria dissociation kit (Miltenyi Biotec) and gentleMACS device (Miltenyi Biotec, program 37C_mLPDK_1)

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

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