Isolation of Colon Cells for Analysis
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Corresponding Organization :
Other organizations : Leibniz Association, University of Regensburg, Johannes Gutenberg University Mainz, University Hospital Regensburg
Variable analysis
- Isolation of cells from colon tissue
- Isolation of T cells from murine tissues
- Clearing of feces from colon
- Preparation of cells according to manufacturer's instructions with the lamina propria dissociation kit (Miltenyi Biotec) and gentleMACS device (Miltenyi Biotec, program 37C_mLPDK_1)
- Positive controls not explicitly mentioned
- Negative controls not explicitly mentioned
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