Fluorescence in situ hybridization was performed as previously described in detail [24 (link)]. Briefly, the tissue sections were directly labeled with the Cyanine 3-conjugated universal bacterial probe EUB338 (5′-GCTGCCTCCCGTAGGAGT-3′, Invitrogen). After probing and hybridization, the slices were restained with 4′,6-diamidino-2-phenylindole. Coimmunostaining with anti-mucin 2 (MUC2) (ab272692, Abcam) was performed.
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