DCFH-DA (Biosharp Co., Ltd., Beijing, China) and flow cytometry were used to detect changes in intracellular ROS levels. Briefly, fresh brain tissues were prepared in a single-cell suspension and then incubated with 10 μM H2DCFDA. The cells were washed and collected for fluorescence detection by flow cytometry.
CAT, SOD, GSH-Px, MDA, IL-1β, TNF-α, and IL-6 levels in the brain and Serum LPS levels in the serum were analyzed with the corresponding kits (Boster, Wuhan, China). The brain tissues were prepared as 10% tissue homogenate in a homogenizer and protein was quantified with a BCA kit (Dalian Meilun Biological Technology Co., Ltd., Dalian, China) [34 (link)].
CAT, SOD, GSH-Px, MDA, IL-1β, TNF-α, and IL-6 levels in the brain and Serum LPS levels in the serum were analyzed with the corresponding kits (Boster, Wuhan, China). The brain tissues were prepared as 10% tissue homogenate in a homogenizer and protein was quantified with a BCA kit (Dalian Meilun Biological Technology Co., Ltd., Dalian, China) [34 (link)].
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