Allo-HCT Transplantation in BALB/c Mice

Allo-HCT was performed as previously described [12 (link),13 (link),15 (link),25 (link),26 (link),27 (link),32 (link)]. Briefly, BALB/c mice were used as recipients and received 9 Gy total body irradiation on day −1, then were transplanted with 5 × 10⁶ T cell-depleted bone marrow (TCD-BM) cells from B6 (CD45.1+) mice along with 0.5 × 10⁶ splenic T cells from B6 (CD45.2+) mice on day 0. Mouse TCD-BM and splenic T cells were prepared using an EasySep mouse CD90.2 positive selection kit II and an EasySep mouse T cell isolation kit (STEMCELL Technologies, Cambridge, MA, USA), respectively.

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Kim S., Ruminski P., Singh M., Staser K., Ashami K., Ritchey J., Lim S., DiPersio J.F, & Choi J. (2024). Novel JAK Inhibitors to Reduce Graft-Versus-Host Disease after Allogeneic Hematopoietic Cell Transplantation in a Preclinical Mouse Model. Molecules, 29(8), 1801.

Publication 2024

EasySep mouse CD90.2 positive selection kit II EasySep mouse T cell isolation kit

Corresponding Organization :

Other organizations : Washington University in St. Louis

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Variable analysis

independent variables

Transplantation of 5 × 10^6 T cell-depleted bone marrow (TCD-BM) cells from B6 (CD45.1+) mice
Transplantation of 0.5 × 10^6 splenic T cells from B6 (CD45.2+) mice

dependent variables

Not explicitly mentioned

control variables

BALB/c mice used as recipients
Recipients received 9 Gy total body irradiation on day -1

controls

Positive control: Not specified
Negative control: Not specified

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