Sixty-six male Sprague-Dawley rats weighing 370±8 g (Japan SLC, Shizuoka, Japan) were maintained at 23°C and under pathogen-free conditions on a 12:12-h dark/light cycle and allowed food and water ad libitum. The present experiments were conducted according to the National Institutes of Health guide for the care and use of Laboratory animals (NIH Publications No. 8023, revised in 1978) and approved by the Animal Research Committee of Kanazawa Medical University (2016–51). Rats were divided into two groups of the shock and control groups: the shock group animals were sensitized with subcutaneous injections of ovalbumin antigen (1 mg, 0.5 ml) and complete Freund’s adjuvant (0.5 ml), as described previously [15 (link), 16 (link)], while the control group animals were injected with adjuvant only without ovalbumin. Two weeks after injection, rats were used for the following experiments.
All drugs were purchased from Sigma Chemical Company (St Louis, MO, USA) and were dissolved in saline. The lot number of the ovalbumin was SLBQ9036V.
All drugs were purchased from Sigma Chemical Company (St Louis, MO, USA) and were dissolved in saline. The lot number of the ovalbumin was SLBQ9036V.
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